![](https://parts.igem.org/images/partbypart/icon_dna.png)
DNA
Part:BBa_J119002:Design
Designed by: Caleb J. Carr Group: Eckdahl_Lab (2011-07-21)
BsaI-GFP2-BsmBI
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1
Illegal PstI site found at 452 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1
Illegal PstI site found at 452 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1
Illegal PstI site found at 452 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1
Illegal PstI site found at 452 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 7
Illegal BsaI.rc site found at 306
Design Notes
This is NOT a BioBrick compatible part. It was designed to solve Hamiltonian Path Problems using multiple genes.
There is a rogue BsaI site at position 304 causing 457 bp to split into 304 bp and 153 bp. It can be removed or altered to lessen the occurrence of additional cutting at that site.
Source
PCR Amplification of GFP gene template split at the sequence ACCC at 351 nt from start.